CD1b Antibody (RIV12) - Azide and BSA Free

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Flow Cytometry: CD1b Antibody (RIV12) - Azide and BSA Free [NBP2-34714] - FCM staining of human PBMCs using CD1b MAb (RIV12).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, CyTOF-ready
Clone
RIV12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

CD1b Antibody (RIV12) - Azide and BSA Free Summary

Immunogen
Human peripheral lymphocytes
Localization
Cell surface
Specificity
The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD1B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • CyTOF-ready
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD1b Antibody (RIV12) - Azide and BSA Free

  • CD1
  • CD1A
  • CD1b antigen
  • CD1B antigen, b polypeptide
  • CD1b molecule
  • CD1b
  • cortical thymocyte antigen CD1B
  • differentiation antigen CD1-alpha-3
  • MGC125990
  • MGC125991
  • R1
  • T-cell surface glycoprotein CD1b

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD1b Antibody (NBP2-34714) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34714AF350
Alexa Fluor 405 NBP2-34714AF405
Alexa Fluor 488 NBP2-34714AF488
Alexa Fluor 532 NBP2-34714AF532
Alexa Fluor 594 NBP2-34714AF594
Alexa Fluor 647 NBP2-34714AF647
Alexa Fluor 700 NBP2-34714AF700
Alexa Fluor 750 NBP2-34714AF750
Allophycocyanin NBP2-34714APC
Biotin NBP2-34714B
DyLight 350 NBP2-34714UV
DyLight 405 NBP2-34714V
DyLight 488 NBP2-34714G
DyLight 550 NBP2-34714R
DyLight 594 NBP2-34714DL594
DyLight 650 NBP2-34714C
DyLight 680 NBP2-34714FR
DyLight 755 NBP2-34714IR
FITC NBP2-34714F
HRP NBP2-34714H
Janelia Fluor 549 NBP2-34714JF549
Janelia Fluor 646 NBP2-34714JF646
PE NBP2-34714PE
PerCP NBP2-34714PCP

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Bioinformatics

Gene Symbol CD1B