CD1a Antibody (HI149) [Alexa Fluor® 700]

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Flow Cytometry: CD1a Antibody (HI149) [Alexa Fluor (R) 700] [NB500-506AF700] - analysis of CD1a in human PBMC. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Image from verified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
HI149
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

CD1a Antibody (HI149) [Alexa Fluor® 700] Summary

Immunogen
Human thymocytes
Localization
Type I membrane protein
Specificity
The antibody HI149 reacts with CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes. HLDA V; WS Code CD01.01
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD1A
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Reviewed Applications
Read 1 Review rated 4
using
NB500-506AF700 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD1a Antibody (HI149) [Alexa Fluor® 700]

  • CD1
  • CD1a antigen
  • CD1A antigen, a polypeptide
  • CD1a molecule
  • CD1a
  • cluster of differentiation 1 A
  • cortical thymocyte antigen CD1A
  • differentiation antigen CD1-alpha-3
  • epidermal dendritic cell marker CD1a
  • FCB6
  • hTa1 thymocyte antigen
  • HTA1
  • R4
  • T6
  • T-cell surface antigen T6/Leu-6
  • T-cell surface glycoprotein CD1a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for CD1a Antibody (NB500-506AF700) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NB500-506AF700:
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Images Ratings Applications Species Date Details
Flow Cytometry CD1a NB500-506AF700
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reviewed by:
Anonymous
Flow Human 11/14/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

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FAQs for CD1a Antibody (NB500-506AF700) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
11/14/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD1A
Entrez