CD19 Antibody (SJ25-C1) [FITC]

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Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-42494
    • Availability
      Product Discontinued

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CD19 Antibody (SJ25-C1) [FITC] Summary

Immunogen
The antibody reacts with the CD19 antigen.
Specificity
The CD19 antigen is the most broadly expressed surface marker for B-cells appearing at the earliest stages of B-cell differentiation. The CD19 antigen is expressed at all stages of B-cell maturation and is lost during terminal B-cell differentiation to plasma cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD19
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 5 ul

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
High grade BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Fluorochrome conjugates should be stored in the dark and direct exposure to light should be avoided during handling and cell staining.

Alternate Names for CD19 Antibody (SJ25-C1) [FITC]

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12

Background

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on CD19.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Bioinformatics

Gene Symbol CD19
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