CD19 Antibody (BC3)

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Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-42248
    • Availability
      Product Discontinued

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CD19 Antibody (BC3) Summary

Immunogen
The antibody reacts with the CD19 antigen (90 kD), which is expressed on all normal B-cells, early B-cells and follicular dendritic cells.
Localization
membrane
Specificity
The antibody reacts with the CD19 antigen (90 kD), which is expressed on all normal B-cells, early B-cells and follicular dendritic cells. The antibody shows a reactivity of 97-100% with cells of several pre-B-cell and B-cell lines. T-cell and monocytic cell lines are negative. In flow cytometrical analysis 70% of human tonsil-, 35% of spleen-, 12% of bone marrow and 10% of peripheral blood lymphocytes are positive. The antibody reacts with non-T acute lymphoblastic leukemia (non T-ALL) and chronic lymphatic leukemia (CLL) cells, whereas T-ALL and acute myelogenous leukemia cells are negative. Antigen location: membrane. Antigen isolated from: chronic Lymphocytes leukemia cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD19
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
This monoclonal antibody can be used for the discrimination of B-cells in tissue and B-cell neoplasms in frozen tissue sections and the identification and separation of B-cells from peripheral bloodsamples by flowcytometrical techniques.For use in immunohistochemistry on frozen sections, fixation with acetone, 10 min., at room temperature is advised. Dilute antibody 1:5-1:20 based on the Indirect Method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilu

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD19 Antibody (BC3)

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12

Background

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on CD19.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Bioinformatics

Gene Symbol CD19
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