CD163 Antibody (GHI/61) [PE]

Images

 
Flow Cytometry: CD163 Antibody (GHI/61) [PE] [NBP2-00078] - Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (open histogram) or Anti-Human CD163 PE (filled histogram). Cells in the ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00078
    • Availability
      Product Discontinued

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CD163 Antibody (GHI/61) [PE] Summary

Immunogen
The immunogen for this antibody was CD163.
Marker
Histiocytic Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD163
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This GHI/61 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD163 Antibody (GHI/61) [PE]

  • CD163 antigenscavenger receptor cysteine-rich type 1 protein M130
  • CD163 molecule
  • CD163
  • GHI/61
  • HbSR
  • Hemoglobin scavenger receptor
  • M130
  • M130macrophage-associated antigen
  • MM130
  • RM3/1

Background

The GHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood. The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163. Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10. Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNF??, can cause shedding of a soluble form of CD163, whose function is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD163
Entrez