CD16/CD32 Antibody (FCR4G8) [FITC]

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Flow Cytometry: CD16/CD32 Antibody (FCR4G8) [FITC] [NB100-63476] - Staining of mouse peritoneal macrophages with RAT ANTI MOUSE CD16/32: FITC.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
FCR4G8
Clonality
Monoclonal
Host
Rat
Conjugate
FITC
Concentration
0.1 mg/ml

CD16/CD32 Antibody (FCR4G8) [FITC] Summary

Immunogen
PU5 1.8 IOE7 Balb/c mouse cell line.
Localization
CD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
Specificity
NB100-63473 recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
FCGR3A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 1.0% BSA
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for CD16/CD32 Antibody (FCR4G8) [FITC]

  • CD16
  • CD16A
  • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • FCG3
  • FCGR3
  • FCGR3A
  • FCGRIII
  • FCR-10
  • FCRIII
  • FCRIIIA
  • IGFR3

Background

The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying Fc gamma R+ cells, monoclonal antibodies to the Fc gamma II/III receptor have been used to block Fc receptor binding of IgG, Fc mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc gamma R for parasitic invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CD16/CD32 Antibody (NB100-63476) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-63473APC
DyLight 350 Labeled NB100-63473UV
DyLight 405 Labeled NB100-63473V
DyLight 488 Labeled NB100-63473G
DyLight 680 Labeled NB100-63473FR
DyLight 755 Labeled NB100-63473IR
PE Labeled NB100-63473PE
PerCP Labeled NB100-63473PCP

Additional CD16/CD32 Products

CD16/CD32 NB100-63476

Bioinformatics Tool for CD16/CD32 Antibody (NB100-63476)

Discover related pathways, diseases and genes to CD16/CD32 Antibody (NB100-63476). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CD16/CD32 Antibody (NB100-63476)

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PTMs for CD16/CD32 Antibody (NB100-63476)

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Blogs on CD16/CD32.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

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Bioinformatics

Gene Symbol FCGR3A