CD155/PVR Knockout 293T Cell Lysate

Images

 
There are currently no images for CD155/PVR Lysate (NBP2-65506).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

CD155/PVR Knockout 293T Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 1
Gene
PVR

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout 293T Cell
Life Stage
Embryonic
Tissue
Kidney

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for CD155/PVR Knockout 293T Cell Lysate

  • CD155 antigen
  • CD155
  • HVED
  • NECL5
  • Necl-5
  • nectin-like 5
  • Nectin-like protein 5
  • poliovirus receptor
  • PVR
  • PVS
  • PVSFLJ25946
  • Tage4

Background

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF6240
Species: Hu
Applications: WB, ELISA, IHC
NBP2-24614
Species: Hu, Mu, Rt, Op, Pm
Applications: WB
NBP2-24763
Species: Hu, Pm
Applications: WB, IHC-P
NBP2-65899
Species: Hu
NBP1-87343
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NBP1-77933
Species: Hu, Mu, Rt, Bv, Ca, Ch, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-36999
Species: Hu, Mu, Rt
Applications: PEP-ELISA
NBP2-65749
Species: Hu
AF666
Species: Hu
Applications: WB, Neut
NBP1-92172
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF2229
Species: Hu
Applications: WB, Simple Western, IHC, ICC
H00005555-P01
Species: Hu
NBP1-56853
Species: Hu
Applications: WB, ICC/IF, IHC
NBP2-01763
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
NBP1-47659
Species: Hu
Applications: WB, IHC, IHC-P
H00005554-R02
Species: Hu
H00002038-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-65506
Species: Hu
Applications: WB

Publications for CD155/PVR Lysate (NBP2-65506) (0)

There are no publications for CD155/PVR Lysate (NBP2-65506).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD155/PVR Lysate (NBP2-65506) (0)

There are no reviews for CD155/PVR Lysate (NBP2-65506). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD155/PVR Lysate (NBP2-65506) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for CD155/PVR Lysate (NBP2-65506)

Discover related pathways, diseases and genes to CD155/PVR Lysate (NBP2-65506). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD155/PVR Lysate (NBP2-65506)

Discover more about diseases related to CD155/PVR Lysate (NBP2-65506).
 

Pathways for CD155/PVR Lysate (NBP2-65506)

View related products by pathway.

PTMs for CD155/PVR Lysate (NBP2-65506)

Learn more about PTMs related to CD155/PVR Lysate (NBP2-65506).
 

Research Areas for CD155/PVR Lysate (NBP2-65506)

Find related products by research area.

Blogs on CD155/PVR

There are no specific blogs for CD155/PVR, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Reg3A Antibody
NBP2-24763

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CD155/PVR Knockout 293T Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol PVR
Entrez