CD15/Lewis X Antibody (MEM-158)

Images

 
Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Analysis using the FITC conjugate of NB500-376. Staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate ...read more
Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Surface staining (mass cytometry) of human peripheral blood cells (after ammonium chloride red blood cell lysis) with anti-human CD15 (MEM-158) 173Yb. Gated ...read more

Product Details

Summary
Reactivity Hu, Bv(-), Po(-), Sh(-)Species Glossary
Applications Flow, IP
Clone
MEM-158
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

CD15/Lewis X Antibody (MEM-158) Summary

Immunogen
Human granulocytes
Localization
Cell Membrane
Specificity
The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. HLDA VI; WS Code AS A053
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
FUT4
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:50

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS (approx. pH 8.0)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for CD15/Lewis X Antibody (MEM-158)

  • CD 15
  • CD15
  • CD-15
  • Lewis X
  • Lewis-X
  • LeX
  • SSEA-1
  • stage-specific embryonic antigen 1

Background

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-376AF405
Alexa Fluor 488 Labeled NB500-376AF488
Alexa Fluor 647 Labeled NB500-376AF647
Alexa Fluor 700 Labeled NB500-376AF700
Allophycocyanin Labeled NB500-376APC
Biotin Labeled NB110-81634
DyLight 350 Labeled NB500-376UV
DyLight 405 Labeled NB500-376V
DyLight 488 Labeled NB500-376G
DyLight 680 Labeled NB500-376FR
DyLight 755 Labeled NB500-376IR
FITC Labeled NB500-377
PE Labeled NB500-376PE
PerCP Labeled NB500-376PCP

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Bioinformatics

Gene Symbol FUT4