CD15/Lewis X Antibody (C3D-1)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
C3D-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD15/Lewis X Antibody (C3D-1) Summary

Immunogen
Purified neutrophils from normal human peripheral blood.
Localization
Cell membrane
Specificity
The specificity of this antibody is similar to antibodies Leu-M1, TU 9 and VIM-D5, recognizing an epitope involving the carbohydrate sequence 3-fucosyl-N-acetyllacetosamine. This antibody reacts with an antigen located on mature granulocytes and on Hodgkin and Reed-Sternberg cells.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FUT4
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:25
Application Notes
IHC-Paraffin requires high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 or Tris-EDTA buffer, pH 9.0 prior to immunostaining.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD15/Lewis X Antibody (C3D-1)

  • CD 15
  • CD15
  • CD-15
  • Lewis X
  • Lewis-X
  • LeX
  • SSEA-1
  • stage-specific embryonic antigen 1

Background

The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FUT4
Entrez
Uniprot