CD14 Antibody (EPR3653)


Western Blot: CD14 Antibody (EPR3653) [NBP1-40683] - (A) PBMC, (B) human tonsil and (C) SW480 cell lysates using a 1:2000 dilution.
Immunohistochemistry-Paraffin: CD14 Antibody (EPR3653) [NBP1-40683] - Tonsil
Immunohistochemistry: CD14 Antibody (EPR3653) [NBP1-40683] - Immunohistochemical analysis of paraffin-embedded human placenta using anti-CD14
Immunohistochemistry-Paraffin: CD14 Antibody (EPR3653) [NBP1-40683] Human tonsil.

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CD14 Antibody (EPR3653) Summary

A synthetic peptide that falls between residues 300-375 in human CD14 was used as an immunogen.
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000-5000
  • Immunocytochemistry/Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-200
Application Notes
In Western blot this antibody detects a band at approximately 53 kDa. This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.)
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP1-40683 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Culture supernatant in buffered aqueous solution
No Preservative
0.003 mg/ml
Protein A or G purified

Alternate Names for CD14 Antibody (EPR3653)

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein


CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD14 Antibody (NBP1-40683)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 2 of 2.
Publications using NBP1-40683 Applications Species
Estruch M, Bancells C, Beloki L et al. CD14 and TLR4 mediate cytokine release promoted by electronegative LDL in monocytes. Atherosclerosis 2013 Aug [PMID: 23880187] (WB, Human) WB Human
Schauer D, Starlinger P, Alidzanovic L, Zajc P. Chemotherapy of Colorectal Liver Metastases Induces a Rapid Rise in Intermediate Blood Monocytes which Predicts Treatment Response. Intermediate monocytes in colorectal cancer therapy. 2016 Mar 22

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD14 Antibody (NBP1-40683). (Showing 1 - 1 of 1 FAQs).

  1. Regarding CD14 Antibody (EPR3653), could you let us know the concentration of sodium azide?
    • This product contains 0.1% sodium azide.

Secondary Antibodies


Isotype Controls

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Gene Symbol CD14