CD14 Antibody (EPR3653)

Images

 
Western Blot: CD14 Antibody (EPR3653) [NBP1-40683] - (A) PBMC, (B) human tonsil and (C) SW480 cell lysates using a 1:2000 dilution.
Immunohistochemistry-Paraffin: CD14 Antibody (EPR3653) [NBP1-40683] - Tonsil
Immunohistochemistry: CD14 Antibody (EPR3653) [NBP1-40683] - Immunohistochemical analysis of paraffin-embedded human placenta using anti-CD14
Immunohistochemistry-Paraffin: CD14 Antibody (EPR3653) [NBP1-40683] Human tonsil.

Product Details

Summary
Product Discontinued
View other related CD14 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-40683
    • Availability
      Product Discontinued

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CD14 Antibody (EPR3653) Summary

Immunogen
A synthetic peptide that falls between residues 300-375 in human CD14 was used as an immunogen.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-5000
  • Immunocytochemistry/Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-200
Application Notes
In Western blot this antibody detects a band at approximately 53 kDa. This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.)
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-40683 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Culture supernatant in buffered aqueous solution
Preservative
No Preservative
Concentration
0.003 mg/ml
Purity
Protein A or G purified

Alternate Names for CD14 Antibody (EPR3653)

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD14 Antibody (NBP1-40683)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP1-40683 Applications Species
Estruch M, Bancells C, Beloki L et al. CD14 and TLR4 mediate cytokine release promoted by electronegative LDL in monocytes. Atherosclerosis 2013 Aug [PMID: 23880187] (WB, Human) WB Human
Schauer D, Starlinger P, Alidzanovic L, Zajc P. Chemotherapy of Colorectal Liver Metastases Induces a Rapid Rise in Intermediate Blood Monocytes which Predicts Treatment Response. Intermediate monocytes in colorectal cancer therapy. 2016 Mar 22

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD14 Antibody (NBP1-40683). (Showing 1 - 1 of 1 FAQs).

  1. Regarding CD14 Antibody (EPR3653), could you let us know the concentration of sodium azide?
    • This product contains 0.1% sodium azide.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD14 Antibody (NBP1-40683)

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Research Areas for CD14 Antibody (NBP1-40683)

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol CD14
Entrez
Uniprot