CD14 Antibody (5A3B11B5)


Western Blot: CD14 Antibody (5A3B11B5) [NB100-2191] - Western blot analysis using COMT Rabbit pAb against HUVE-12 (1), A431 (2), K562 (3), HepG2 (4) cell lysate and spermary tissue (5) lysate.
Immunohistochemistry-Paraffin: CD14 Antibody (5A3B11B5) [NB100-2191] - Analysis of human lymphnode, showing membrane localization using CD14 mouse mAb with DAB staining.
Flow Cytometry: CD14 Antibody (5A3B11B5) [NB100-2191] - Analysis of human PBMC using CD14 mouse mAb.
Immunohistochemistry-Paraffin: CD14 Antibody (5A3B11B5) [NB100-2191] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left), ovary cancer tissues (right) using COMT Rabbit pAb with DAB staining.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
0.4 mg/ml

Order Details

CD14 Antibody (5A3B11B5) Summary

Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).
CD14 (5A3B11B5)
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide
0.4 mg/ml
Protein A or G purified


  • Western Blot 1:100 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500

Alternate Names for CD14 Antibody (5A3B11B5)

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein


CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Product PDFs


Gene Symbol CD14

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