CD14 Antibody (5A3B11B5)

Images

 
Western Blot: CD14 Antibody (5A3B11B5) [NB100-2191] - Western blot analysis using COMT Rabbit pAb against HUVE-12 (1), A431 (2), K562 (3), HepG2 (4) cell lysate and spermary tissue (5) lysate.
Immunohistochemistry-Paraffin: CD14 Antibody (5A3B11B5) [NB100-2191] - Analysis of human lymphnode, showing membrane localization using CD14 mouse mAb with DAB staining.
Flow Cytometry: CD14 Antibody (5A3B11B5) [NB100-2191] - Analysis of human PBMC using CD14 mouse mAb.

Product Details

Summary
Product Discontinued
View other related CD14 Primary Antibodies

Order Details


    • Catalog Number
      NB100-2191
    • Availability
      Product Discontinued

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CD14 Antibody (5A3B11B5) Summary

Immunogen
Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).
Specificity
CD14 (5A3B11B5)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Positive Control
CD14 Lysate (NBL1-08905)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
0.4 mg/ml
Purity
Protein A or G purified

Alternate Names for CD14 Antibody (5A3B11B5)

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD14 Antibody (NB100-2191)

Discover related pathways, diseases and genes to CD14 Antibody (NB100-2191). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for CD14 Antibody (NB100-2191)

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Research Areas for CD14 Antibody (NB100-2191)

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol CD14
Entrez