CD133 Antibody


Western Blot: CD133 Antibody [NBP2-44247] - Total protein from A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 more
Immunocytochemistry/ Immunofluorescence: CD133 Antibody [NBP2-44247] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were more
Flow (Intracellular): CD133 Antibody [NBP2-44247] - Fixed and permeabilized A431 cells were stained at a 1:1000 dilution with hCD133 and detected using a Gt x ms DyLight 488 secondary (blue) shown with secondary only more
Western Blot: CD133 Antibody [NBP2-44247] - Total human fetal fetal liver protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF
1.0 mg/ml

CD133 Antibody Summary

A synthetic peptide made to an N-terminal portion of the human CD133 protein (between residues 1-100) [UniProt# O43490]
Immunogen has 100% homology to all isoforms of human CD133.
Immunogen affinity purified
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  • Western Blot 2 ug/ml
  • Flow Cytometry 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Flow (Intracellular) 1:1000

Reactivity Notes

This antibody is not expected to react with mouse or rat due to poor homology.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for CD133 Antibody

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1


Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for CD133 Antibody (NBP2-44247): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD133 Antibody (NBP2-44247) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-44247AF488
Alexa Fluor 647 Labeled NBP2-44247AF647
Allophycocyanin Labeled NBP2-44247APC
Biotin Labeled NBP2-44247B
DyLight 350 Labeled NBP2-44247UV
DyLight 405 Labeled NBP2-44247V
DyLight 488 Labeled NBP2-44247G
DyLight 550 Labeled NBP2-44247R
DyLight 650 Labeled NBP2-44247C
DyLight 680 Labeled NBP2-44247FR
DyLight 755 Labeled NBP2-44247IR
HRP Labeled NBP2-44247H
PE Labeled NBP2-44247PE
PerCP Labeled NBP2-44247PCP

Additional Array Products

Array NBP2-44247

Bioinformatics Tool for CD133 Antibody (NBP2-44247)

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Diseases for CD133 Antibody (NBP2-44247)

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Blogs on CD133.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Gene Symbol PROM1