CD133 Antibody

Images

 
Western Blot: CD133 Antibody [NBP2-44247] - Western Blot image of anti-human CD133. Total protein from A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunocytochemistry/ Immunofluorescence: CD133 Antibody [NBP2-44247] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Flow (Intracellular): CD133 Antibody [NBP2-44247] - Fixed and permeabilized A431 cells were stained at a 1:1000 dilution with hCD133 and detected using a Gt x ms Dylight 488 secondary (blue) shown with secondary only ...read more
Western Blot: CD133 Antibody [NBP2-44247] - Western Blot image of anti-human CD133. Total human fetal fetal liver protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD133 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human CD133 protein (between residues 1-100) [UniProt O43490]
Specificity
Immunogen has 100% homology to all isoforms of human CD133.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PROM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Flow (Intracellular) 1:1000

Reactivity Notes

This antibody is not expected to react with mouse or rat due to poor homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD133 Antibody

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for CD133 Antibody (NBP2-44247): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD133 Antibody (NBP2-44247) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-44247AF488
Alexa Fluor 647 Labeled NBP2-44247AF647
Allophycocyanin Labeled NBP2-44247APC
Biotin Labeled NBP2-44247B
DyLight 350 Labeled NBP2-44247UV
DyLight 405 Labeled NBP2-44247V
DyLight 488 Labeled NBP2-44247G
DyLight 550 Labeled NBP2-44247R
DyLight 650 Labeled NBP2-44247C
DyLight 680 Labeled NBP2-44247FR
DyLight 755 Labeled NBP2-44247IR
HRP Labeled NBP2-44247H
PE Labeled NBP2-44247PE
PerCP Labeled NBP2-44247PCP

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CD133 NBP2-44247

Bioinformatics Tool for CD133 Antibody (NBP2-44247)

Discover related pathways, diseases and genes to CD133 Antibody (NBP2-44247). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD133.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

CD133
Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Bioinformatics

Gene Symbol PROM1
Entrez