CD133 Antibody (217106) [Unconjugated]

D3 mouse cells were stained with Rat Anti-Mouse CD133 Monoclonal Antibody (Catalog # MAB11332, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

CD133 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD133 Monoclonal Antibody (Catalog # MAB11332) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in epithelial cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 217106
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CD133 Antibody (217106) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD133
  Accession # O54990
• Specificity: Detects mouse CD133 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD133 Antibody (217106) [Unconjugated]

• AC133 Stargardt disease 4 (autosomal dominant)
• AC133
• CD133 retinal 2
• CD133
• PROM1
• prominin (mouse)-like 1
• Prominin 1
• PROML1

Background

Mouse Prominin-1 (also known as CD133) is a 867 aminoacids glycoprotein encoded by the PROM1 gene. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM. In human, Prom-1 is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. In Mouse, at least 7 different isoforms have been proposed. Human and mouse PROM-1 share only 61% aminoacid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O54990()