CD11c Antibody (N418)

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Flow Cytometry: CD11c Antibody (N418) [NBP1-43441] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43441. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.125 ug of Armenian Hamster IgG ...read more
Flow Cytometry: CD11c Antibody (N418) [NBP1-43441] - Analysis using the Allophycocyanin conjugate of NBP1-43441. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) PE and staining buffer ...read more
Flow Cytometry: CD11c Antibody (N418) [NBP1-43441] - Analysis using the Biotin conjugate of NBP1-43441. Staining of C57BL/6 splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or ...read more
Flow Cytometry: CD11c Antibody (N418) [NBP1-43441] - Analysis using the FITC conjugate of NBP1-43441. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.125 ug of Armenian Hamster IgG Isotype ...read more
Flow Cytometry: CD11c Antibody (N418) [NBP1-43441] - Analysis using the PE conjugate of NBP1-43441. Staining of mouse splenocytes with Anti-Mouse CD11c FITC (left) and PE (right), and Anti-Human/Mouse CD45R (B220) APC. ...read more
Flow Cytometry: CD11c Antibody (N418) [NBP1-43441] - Analysis using the PE/Cy5 conjugate of NBP1-43441. Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.25 ug of Armenian Hamster IgG Isotype ...read more
Flow Cytometry: CD11c Antibody (N418) [NBP1-43441] - Analysis using the PE/Cy5.5 conjugate of NBP1-43441. Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.25 ug of Anti-Mouse CD11c PE-Cy5.5. ...read more
Flow Cytometry: CD11c Antibody (N418) [NBP1-43441] - Analysis using the PE/Cy7 conjugate of NBP1-43441. Staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.25 micrograms of Armenian Hamster IgG ...read more

Product Details

Summary
Product Discontinued
View other related CD11c Primary Antibodies

Order Details


    • Catalog Number
      NBP1-43441
    • Availability
      Product Discontinued

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CD11c Antibody (N418) Summary

Immunogen
The immunogen for this antibody was CD11c.
Marker
Dendritic Cell Marker
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
ITGAX
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The N418 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Publications
Read Publication using
NBP1-43441 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD11c Antibody (N418)

  • 95 alpha chain
  • 95
  • 95, alpha subunit
  • CD11 antigen-like family member C
  • CD11c antigen
  • CD11c
  • CD11Cleu M5, alpha subunit
  • Integrin alpha X
  • integrin alpha-X
  • integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
  • integrin, alpha X (complement component 3 receptor 4 subunit)
  • ITGAX
  • Leu M5
  • Leukocyte adhesion glycoprotein p150
  • Leukocyte adhesion receptor p150
  • leukocyte surface antigen p150
  • myeloid membrane antigen, alpha subunit
  • p150 95 integrin alpha chain
  • p150,95 alpha
  • SLEB6

Background

The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11c Antibody (NBP1-43441)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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Secondary Antibodies

 

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Blogs on CD11c.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

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Bioinformatics

Gene Symbol ITGAX
Entrez