CD117/c-kit Antibody

Images

 
Western Blot: CD117/c-kit Antibody [NBP1-19865] - Extracts from A549 cells.
Immunohistochemistry: CD117/c-kit Antibody [NBP1-19865] - Analysis of rat heart section using anti-c-Kit antibody. Image from verified customer review.

Product Details

Summary
Product Discontinued
View other related CD117/c-kit Primary Antibodies

Order Details


    • Catalog Number
      NBP1-19865
    • Availability
      Product Discontinued

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CD117/c-kit Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 681-730 of Human c-Kit.
Marker
Hematopoietic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Western blot (WB) analyzes of c-Kit (S715)pAb in extracts from A549 cells. Immunohistochemistry was reported in scientific literature. Use in Immunohistochemistry-paraffin and in vitro assay reported in scientific literature (PMID 24655344)
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-19865 in the following applications:

Publications
Read Publications using
NBP1-19865 in the following applications:

  • 4 publications
  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (approx. pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD117/c-kit Antibody

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

The c-Kit proto-oncogene is a member of the receptor tyrosine kinase family and, more specifically, is closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene (v-Kit), encodes a transmembrane receptor. c-Kit regulates a variety of biological responses including chemotaxis, cell proliferation, apoptosis and adhesion. c-Kit is also identical with the product of the W locus in mice and, as such, is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and is encoded at the murine steel (SI) locus. Kit is the human homolog of the protooncogene c-Kit. Mutations in Kit are integral for tumor growth and progression in various cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD117/c-kit Antibody (NBP1-19865)(5)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(4)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Rat
(3)
All Species

Review for CD117/c-kit Antibody (NBP1-19865) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-19865:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence CD117/c-kit NBP1-19865
Enlarge
reviewed by:
Zheng xin
IF Rat 10/08/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedrat heart
SpeciesRat
LotCA36131
Commentsdo more experiment

Blocking

Blocking DetailsPBS, 10min, room temperature

Primary Anitbody

Dilution RatioPBS,4 degree overnight

Secondary Antibody

Secondary DescriptionGoat anti-rabbit CY3
Secondary Manufacturer Cat#proteintech Sa00009-2
Secondary Concentration1:300

Details

Detection Notesolympus IX81, 200ms
Fixation Detailsparaformaldehyde, Tween 20, PBS, 10min, room temperature
Wash DescriptionPBS, 10min, 3 times

Comments

Commentsdo more experiment

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD117/c-kit Antibody (NBP1-19865). (Showing 1 - 1 of 1 FAQs).

  1. Here a customer intends to buy our c-Kit Antibody 0.1 mg Hematopoietic Stem Cell Marker,the catalog is NBP1-1986.He wonders the Isotype of it.
    • Our c-Kit antibody with catalogue number NBP1-19865 is a rabbit polyclonal and of the isotype IgG.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD117/c-kit Antibody (NBP1-19865)

Discover related pathways, diseases and genes to CD117/c-kit Antibody (NBP1-19865). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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PTMs for CD117/c-kit Antibody (NBP1-19865)

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Research Areas for CD117/c-kit Antibody (NBP1-19865)

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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Zheng xin
10/08/2014
Application: IF
Species: Rat

Bioinformatics

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