CD117/c-kit Antibody Pair [HRP]

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Sandwich ELISA: CD117/c-kit Antibody Pair [HRP] [NBP2-79381] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP, PLA, S-ELISA, WB (-)
Clonality
Monoclonal
Conjugate
HRP
Standard Curve Range
23.44-1500 pg/ml (example only; lot dependent)
Sensitivity
23.4375 pg/ml (example only; lot dependent)

Order Details

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Catalog# & Conjugate Size Price

CD117/c-kit Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human CD117/c-kit.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD117/c-kit.
Standard Curve Range
23.44-1500 pg/ml (example only; lot dependent)
Sensitivity
23.4375 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Gene
KIT

Applications/Dilutions

Dilutions
  • Immunoprecipitation 5-10 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
  • Sandwich ELISA
Application Notes
1. Capture Antibody (NBP2-90036): Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5 ng/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 0.2 mg/ml of rabbit anti-CD117/c-kit monoclonal antibody (in PBS, pH 7.4).
  2. Detection Antibody: 0.2 mg/ml mouse anti-CD117/c-kit monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4).
  3. Standard: Each vial contains 90 ng of recombinant CD117/c-kit.

Alternate Names for CD117/c-kit Antibody Pair [HRP]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.

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