CCR2 Antibody

Product Discontinued

CCR2 Antibody Summary

• Immunogen: Synthetic peptide taken within amino acid region 20-70 on human CCR2 protein.
• Specificity: This antibody does not cross react to other chemokine receptors.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunoprecipitation 1:200
  • Western Blot 1:500
• Application Notes: Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 30098312).

Reactivity Notes

The CCR2 peptides sequences were conserved in human, gibbon, gorilla but not in other species. Rat reactivity reported in scientific literature (PMID: 30098312).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CCR2 Antibody

• C-C chemokine receptor type 2
• C-C CKR-2
• CC-CKR-2
• CC-CKR-2CKR2B
• CCR2
• CCR-2
• CCR2A
• CCR2B
• CD192 antigen
• CD192
• chemokine (C-C motif) receptor 2
• CKR2
• CKR2A
• CMKBR2
• CMKBR2MGC111760
• FLJ78302
• MCP-1 receptor
• MCP-1-R
• MCP-1-RMGC103828
• MGC168006
• Monocyte chemoattractant protein 1 receptor
• monocyte chemotactic protein 1 receptor

Background

Chemokine receptors represent a subfamily of ~20 GPCRs that were originally identified by their roles in immune cell trafficking. Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and RANTES, members of the beta chemokine family of leukocyte chemo-attractants, bind to a common seven-transmembrane-domain human receptor. Chemokine (Chemo-attractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CCR) belong to the superfamily of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and directing the proliferation and migration of immature neurons, glia and their precursors (1). Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases. Induction of chemokines and infiltration of chemokine receptor-bearing cells has also been shown in a variety of animal models of renal diseases, as well as in human diseases and allograft rejection (2) Monocyte activation protein 1 (MACP1) or CCL2 is the ligand for CCR2, upon activation CCR2 mediate robust release of Ca+2 and monocytic infilteration of tissues in atherosclerosis and other inflammatory diseases. The CCR2 receptors are most closely related to MCP1/CCR1/RANTES receptors.CCR2 receptor are also implicated in several neuropathological syndromes including neuropathic pain (3). CCR2 and CXCR3 receptors also mediate leukocyte adhesion and migration in to acute kidney injury caused by ischemia-reperfusion. CCR2 is a multi-pass membrane protein, containing 7TMDs with apparent MW of 46kDa (373 aa) expressed on many different blood cells. The CCR2 protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins. The short carboxy terminal is intracellular and has putative post-translational sites. The Anti-CCR2-selective antibodies were generated against conserved but unique sequences from N-terminal region of human CCR2 receptor proteins. The peptide sequence resides between aa 30-60 of CCR2 and are unique to CCR2 protein. Antibodies generated against this peptide are specific for CCR2, these antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. The affinity purified antibodies are also conjugated to FITC and biotin for direct applications in IHC and cell sorting experiments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR2 Antibody (NBP1-78169)(3)

Publications using NBP1-78169

• Rivera P, Fernandez-Arjona MDM, Silva-Pena D et al. Pharmacological blockade of fatty acid amide hydrolase (FAAH) by URB597 improves memory and changes the phenotype of hippocampal microglia despite ethanol exposure Biochem. Pharmacol. 2018-08-08 [PMID: 30098312] (IHC-FrFl, Rat)
• Tran PB, Miller RJ. Chemokine receptors: signposts to brain development and disease. Nat Rev Neurosci 2003-01-01 [PMID: 12778117]
  
  Details:
  This citation used the FITC version of this antibody.
• Segerer S. Am J Kidney Dis. 41, :S15-8. 2003-01-01 [PMID: 12612944]

Bioinformatics

• Gene Symbol: CCR2
• Entrez:
  • 729230(Human)
  • 12772(Mouse)
  • 60463(Rat)
• Uniprot:
  • P41597(Human)