CCL4/MIP-1 beta Antibody [Biotin] Summary
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP1 beta protein.
Delipidation and Defibrination
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- Western Blot 1:2000-1:10000
- ELISA 1:20000-1:100000
Human MIP-1 beta Antibody Biotin Conjugated has been tested for use in ELISA and western blotting.Expect a band approximately 15 kDa in size corresponding - human MIP-1Beta protein by western blotting in the appropriate cell lysate or extract. Immunology Research
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01% Sodium Azide
Delipidation and Defibrination
Alternate Names for CCL4/MIP-1 beta Antibody [Biotin]
- C-C motif chemokine 4
- chemokine (C-C motif) ligand 4
- G-26 T-lymphocyte-secreted protein
- Lymphocyte activation gene 1 protein
- Macrophage inflammatory protein 1-beta
- MIP1 beta
- MIP-1 beta
- MIP-1 beta/CCL4
- PAT 744
- Protein H400
- secreted protein G-26
- small inducible cytokine A4 (homologous to mouse Mip-1b)
- Small-inducible cytokine A4
- T-cell activation protein 2
MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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