CCL2/MCP1 Antibody

Product Discontinued

CCL2/MCP1 Antibody Summary

• Immunogen: E. coli expressed rat MCP1.
• Localization: Secreted
• Specificity: Reacts to mouse JE and rat MCP1
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000

Reactivity Notes

Cross-reacts with Mouse JE and Rat MCP-1.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Purity: Protein A purified

Alternate Names for CCL2/MCP1 Antibody

• C-C motif chemokine 2
• chemokine (C-C motif) ligand 2
• GDCF-2
• HC11Monocyte secretory protein JE
• MCAFMonocyte chemotactic and activating factor
• MCP1 Monocyte chemotactic protein 1
• MCP-1 Small-inducible cytokine A2
• MGC9434
• Monocyte chemoattractant protein 1
• monocyte chemoattractant protein-1
• SCYA2HSMCR30
• small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
• small inducible cytokine subfamily A (Cys-Cys), member 2
• SMC-CF

Background

Rat MCP-1/JE (monocyte chemoattracctant protein-1) is a 148-amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library1. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3'-end. This 3'-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and explains for the higher molecular weight (25 kDa)2. In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL2/MCP1 Antibody (NB110-2000)(2)

Publications using NB110-2000

• Uluer ET, Vatansever HS, Aydede H, Ozbilgin MK. Keratinocytes derived from embryonic stem cells induce wound healing in mice. Biotech Histochem. 2018-11-21 [PMID: 30460873] (IHC-P, Mouse)
• Lund AW, Wagner M, Fankhauser M et al. Lymphatic vessels regulate immune microenvironments in human and murine melanoma. J. Clin. Invest. 2016-09-01 [PMID: 27525437] (WB, Mouse)

Bioinformatics

• Gene Symbol: CCL2
• Entrez:
  • 20296(Mouse)
  • 24770(Rat)
• Uniprot:
  • P14844()