Caspr2 Antibody (S67-25)

Images

 
Western Blot: Caspr2 Antibody (S67-25) [NBP1-49575] - Lane 1: HEK293T control cell lysate and Lane2: Caspr2 plasmid transfected HEK293T cell lysate. Image from verified customer review.
Immunohistochemistry-Frozen: Caspr2 Antibody (S67-25) [NBP1-49575] - in frozen sections of mouse brain extract using this antibody.
Western Blot: Caspr2 Antibody (S67-25) [NBP1-49575] - Rat brain membrane tissues using a 1:1000 dilution.

Product Details

Summary
Product Discontinued
View other related Caspr2 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-49575
    • Availability
      Product Discontinued

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Caspr2 Antibody (S67-25) Summary

Immunogen
Fusion protein 96-1265 (extracellular domain) of human CASPR2, accession number NP_054860.
Isotype
IgG2 Alpha
Clonality
Monoclonal
Host
Mouse
Gene
CNTNAP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP1-49575 in the following applications:

Publications
Read Publications using NBP1-49575.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Caspr2 Antibody (S67-25)

  • AUTS15
  • Caspr2
  • CASPR2DKFZp781D1846
  • CDFE
  • Cell recognition molecule Caspr2
  • CNTNAP2
  • contactin associated protein-like 2
  • contactin-associated protein 2
  • contactin-associated protein-like 2
  • homolog of Drosophila neurexin IV
  • KIAA0868
  • NRXN4
  • NRXN4Caspr2
  • PTHSL1

Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Caspr (contactin-associated protein) is a part of the neurexin family. It lies in the paranodal section of the myelin sheath. It's role is for myelin sheath attachment along with contactin in a cis-complex (3). Caspr and Caspr2 regulate the formation of distinct axonal domains around the nodes of Ranvier. Caspr is required for the generation of a membrane barrier at the paranodal junction, whereas Caspr serves as a membrane scaffold that clusters Kv1 channels at the juxtaparanodal region. Both interact with protein 4.1B (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspr2 Antibody (NBP1-49575)(2)

Reviews for Caspr2 Antibody (NBP1-49575) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 application: IF, WB.

Reviews using NBP1-49575:
Filter by Applications
IF
(2)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Fugui Li
IF 10/20/2014
View

Summary

ApplicationImmunofluorescence
CommentsThe antibody didn't work under unpermeablized condition, it should be working because it targets the extracelluar domain of Caspr2 protein, however it works after the transfected cells were permeablized with 0.2% Triton in PBS for 5 minutes.
Western Blot Caspr2 NBP1-49575
Enlarge
reviewed by:
Alissa Taylor
WB Human 10/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedCaspr2 plasmid transfected HEK293T cell lysate
SpeciesHuman
 
reviewed by:
YONGJIE LAI
IF Human 09/24/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedCaspr2 plasmid transfected HEK293T cells
SpeciesHuman

Blocking

Blocking Details5% donkey serum in PBS

Primary Anitbody

Dilution Ratio1:200 dilution in culture media 1 hour at 37 degrees

Secondary Antibody

Secondary DescriptionTritc-Donkey anti-mouse IgG
Secondary Concentration1:200

Details

Wash DescriptionPBS 3X

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Caspr2 Antibody (NBP1-49575) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for Caspr2 Antibody (NBP1-49575)

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Calculators

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Fugui Li
10/20/2014
Application: IF
Species:

Alissa Taylor
10/01/2014
Application: WB
Species: Human

 
YONGJIE LAI
09/24/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol CNTNAP2
Entrez
Uniprot