Recombinant Human Caspase-9 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, HPLC
Concentration
Lyoph

Order Details

Recombinant Human Caspase-9 Protein Summary

Description
Recombinant protein for Human Caspase-9

Source:E. coli.
Amino Acid Sequence:(P55211)

Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
CASP9
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Unit Definition: One unit of the recombinant caspase-9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
Specific activity: > 400 units/mg

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Semi Dry powder
Concentration
Lyoph
Purity
>90%, by SDS-PAGE
Reconstitution Instructions
Reconstitute to 1 unit per l in PBS containing 15% glycerol.

Alternate Names for Recombinant Human Caspase-9 Protein

  • APAF-3APAF3
  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • Cas9
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • Caspase-9
  • EC 3.4.22.62
  • ICE-LAP6CASPASE-9c
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease

Background

Caspase-9 is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis procaspase-9 is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger activation of downstream effector caspases, leading to disassembly of cell structures. The recombinant active human caspase-9 was expressed in E. coli. The active caspase-9 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-LEHD-pNA (Cat. #1076-200) or Ac-LEHD-AFC (1075-200).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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FAQs for Caspase-9 Protein (NBP1-99606). (Showing 1 - 1 of 1 FAQ).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

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Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
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Bioinformatics

Gene Symbol CASP9
Entrez