Caspase-9 Antibody (10-1-87)

Images

 
Western Blot: Caspase 9 Antibody (10-1-87) [NBP1-19195] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NBP1-19195) at 1:500.
Western Blot: Caspase-9 Antibody [NBP1-19195] - MW marker (1), Jurkat (2), and Jurkat + staurosporine (3) cells probed with Caspase 9 Monoclonal Antibody (10-1-87)

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-19195
    • Availability
      Product Discontinued

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Caspase-9 Antibody (10-1-87) Summary

Immunogen
Recombinant full length human Caspase-9
Specificity
Caspase-9 (10-1-87)
Isotype
IgG2
Clonality
Monoclonal
Host
Mouse
Gene
CASP9
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
Sodium Azide
Purity
Protein G purified

Alternate Names for Caspase-9 Antibody (10-1-87)

  • APAF-3APAF3
  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • Cas9
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • Caspase-9
  • EC 3.4.22.62
  • ICE-LAP6CASPASE-9c
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease

Background

The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. Caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-19195
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Applications: WB

Publications for Caspase-9 Antibody (NBP1-19195) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Caspase-9 Antibody (NBP1-19195). (Showing 1 - 1 of 1 FAQ).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Secondary Antibodies

 

Isotype Controls

Additional Caspase-9 Products

Bioinformatics Tool for Caspase-9 Antibody (NBP1-19195)

Discover related pathways, diseases and genes to Caspase-9 Antibody (NBP1-19195). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Caspase-9 Antibody (NBP1-19195)

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Pathways for Caspase-9 Antibody (NBP1-19195)

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PTMs for Caspase-9 Antibody (NBP1-19195)

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Research Areas for Caspase-9 Antibody (NBP1-19195)

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Blogs on Caspase-9.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Bioinformatics

Gene Symbol CASP9
Entrez