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May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
- In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
Positive Control Lysate(s)
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