Caspase-7 Antibody

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Western Blot: Caspase-7 Antibody [NBP1-45442] - Analysis of Jurkat cells induced with 2 mM of Camptothecin (1039-1) for 6 hours.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Caspase-7 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 195 of human caspase-7 (BAG61059).
Specificity
The antibody detects only the large subunit of cleaved caspase-7. The antibody does not recognize full length of caspase-7 or other caspases.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody detects only the large subunit of cleaved caspase-7. The antibody does not recognize full length of caspase-7 or other caspases.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1.0% BSA and 50% Glycerol
Preservative
0.02% Thimerosal
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-7 Antibody

  • apoptosis-related cysteine protease
  • Apoptotic protease Mch-3
  • CASP7
  • CASP-7
  • caspase 7, apoptosis-related cysteine peptidase
  • Caspase7
  • Caspase-7
  • EC 3.4.22
  • EC 3.4.22.60
  • ICE-like apoptotic protease 3
  • Lice2 alpha/beta/gamma
  • Mch3

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-7 is derived from the cleavage of proMCH3-alpha into 2 subunits, p20 and p12 by Caspase-3. Sequence analysis revealed that the MCH3-alpha amino acid sequence had the highest homology to caspase-3 (53% identity) and less than 37% identity with all other family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-7.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP7
Entrez
Uniprot