Caspase-6 Antibody

Images

 
Western Blot: Caspase-6 Antibody [NBP2-15718] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NBP2-15718] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.77 mg/ml

Order Details

Caspase-6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Caspase 6. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.77 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-6 Antibody

  • Apoptotic protease Mch-2
  • CASP6
  • CASP-6
  • caspase 6, apoptosis-related cysteine peptidase
  • caspase 6, apoptosis-related cysteine protease
  • Caspase6
  • Caspase-6
  • EC 3.4.22
  • Mch2
  • MCH2EC 3.4.22.59

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CASP6
Entrez
Uniprot