Caspase-4 Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-10447
    • Availability
      Product Discontinued

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Caspase-4 Antibody Summary

Immunogen
Synthetic peptide: KQISPNKKAHPNMEAGPPE conjugated to KLH, corresponding to amino acids 81-98 of Human Caspase 4 (with N-terminally added K).
Localization
Cytoplasmic and Mitochondrial
Specificity
Anti-Caspase 4 specifically recognizes human procaspase 4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunoprecipitation
  • Microarray
Application Notes
IP: Use 5-10 ug of antibody to IP Caspase-4 from 250-300 ug RIPA lysate of human ECV304 endothelioid cells. WB: Use at a dilution of 1/250. Detects a band of approximately 43-47 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
Caspase-4 Lysate (NBL1-08712)

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4) and 1.0% BSA
Preservative
15mM Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Caspase-4 Antibody

  • apoptotic cysteine protease Mih1/TX
  • CASP4
  • CASP-4
  • caspase 4, apoptosis-related cysteine peptidase
  • caspase 4, apoptosis-related cysteine protease
  • Caspase4
  • Caspase-4
  • EC 3.4.22
  • EC 3.4.22.57
  • ICE(rel)II
  • ICE(rel)-II
  • ICEREL-II
  • ICH2
  • ICH-2
  • Mih1/TX
  • Protease ICH-2
  • Protease TX
  • TX

Background

Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67% homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Caspase-4 Antibody (NB120-10447)

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Blogs on Caspase-4.

Caspase-4 - a human protease with roles in inflammation and immunity
Caspases are a family of cysteine-aspartic acid proteases that cleave caspase proenzymes as well as other protein substrates. Caspases are well known for their role in apoptosis, but they also play a significant role in other cellular processes inc...  Read full blog post.

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Bioinformatics

Gene Symbol CASP4
Entrez