Recombinant Mouse Caspase-3 Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-99922
    • Availability
      Product Discontinued

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Recombinant Mouse Caspase-3 Protein Summary

Description
A recombinant protein corresponding to Mouse Caspase-3
Preparation
Method
>90% SDS-PAGE
Protein/Peptide Type
Recombinant Protein
Gene
CASP3
Purity
>90% SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Concentration
Lyoph
Purity
>90% SDS-PAGE
Reconstitution Instructions
Reconstitute with PBS containing 15% glycerol to a final concentration of 0.1 mg/ml.

Alternate Names for Recombinant Mouse Caspase-3 Protein

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase-3 (also know as CPP32, Yama and apopain) is a member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. Proteolytic processing of this inactive proenzyme generates the 17 kD and 12 kD subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be key signal-transducing events in apoptosis. Procaspase-3, like other procaspases with short N-terminal prodomains, is considered to be a downstream or "effector" enzyme. Pro-caspase-3 can be cleaved and activated by caspase-8 and -10 which are themselves activated through interaction with death ligand receptor/adapter protein complexes (e.g., Fas/FADD). Procaspase-3 is also processed by caspase-9, the apical caspase that is activated by binding to the Apaf-1/cytochrome c/ATP "apoptosome". Thus, activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c.The recombinant Procaspase-3 is produced by expression of a human cDNA in E. coli. The purified protein is highly activatable by treatment with active recombinant caspase-8. We routinely test the activation using 10-30 ng of the procaspase-3 treated with 0.1-0.5 units of the active recombinant caspase-8 in 100ul of Reaction Buffer for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric and Colorimetric Assay Kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CASP3
Entrez