Recombinant Mouse Caspase-3 Protein Summary
A recombinant protein corresponding to Mouse Caspase-3
| Protein/Peptide Type
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Reconstitute with PBS containing 15% glycerol to a final concentration of 0.1 mg/ml.
Alternate Names for Recombinant Mouse Caspase-3 Protein
- apoptosis-related cysteine protease
- caspase 3, apoptosis-related cysteine peptidase
- CPP32SREBP cleavage activity 1
- Cysteine protease CPP32
- EC 3.4.22
- EC 18.104.22.168
- PARP cleavage protease
- Protein Yama
Caspase-3 (also know as CPP32, Yama and apopain) is a member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. Proteolytic processing of this inactive proenzyme generates the 17 kD and 12 kD subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be key signal-transducing events in apoptosis. Procaspase-3, like other procaspases with short N-terminal prodomains, is considered to be a downstream or "effector" enzyme. Pro-caspase-3 can be cleaved and activated by caspase-8 and -10 which are themselves activated through interaction with death ligand receptor/adapter protein complexes (e.g., Fas/FADD). Procaspase-3 is also processed by caspase-9, the apical caspase that is activated by binding to the Apaf-1/cytochrome c/ATP "apoptosome". Thus, activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c.The recombinant Procaspase-3 is produced by expression of a human cDNA in E. coli. The purified protein is highly activatable by treatment with active recombinant caspase-8. We routinely test the activation using 10-30 ng of the procaspase-3 treated with 0.1-0.5 units of the active recombinant caspase-8 in 100ul of Reaction Buffer for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric and Colorimetric Assay Kits.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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