Caspase-3 Antibody

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Western Blot: Caspase-3 Antibody [NBP2-15716] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: Caspase 3 Antibody [NBP2-15716] - Caspase 3 antibody detects CASP3 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody [NBP2-15716] - Paraffin-embedded rat liver. Caspase 3 antibody diluted at 1:500.
Western Blot: Caspase-3 Antibody [NBP2-15716] - Wild-type (WT) and Caspase 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody. HRP-conjugated ...read more
Western Blot: Caspase-3 Antibody [NBP2-15716] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody. ...read more
Western Blot: Caspase-3 Antibody [NBP2-15716] - Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Un-treated (-) and treated (+), 30 uM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 ...read more
Western Blot: Caspase-3 Antibody [NBP2-15716] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody diluted at 1:1000. The ...read more
Western Blot: Caspase-3 Antibody [NBP2-15716] - A. 30 ug C8D30 whole cell extract B. 30 ug NIH-3T3 whole cell extract C. 30 ug Raw264.7 whole cell extract D. 30 ug C2C12 whole cell extract 12% SDS-PAGE Caspase 3 ...read more
Western Blot: Caspase-3 Antibody [NBP2-15716] - A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 12% SDS-PAGE Caspase 3 antibody dilution: ...read more
Western Blot: Caspase-3 Antibody [NBP2-15716] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract C. 30 ug HL-60 whole cell lysate/extract 12% SDS-PAGE Caspase 3 antibody dilution: 1:5000 ...read more
Immunohistochemistry-Paraffin: Caspase 3 Antibody [NBP2-15716] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: Caspase-3 Antibody [NBP2-15716] - HeLa whole cell extracts. Analysis was performed using Caspase 3 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.54 mg/ml
   

Biological Strategies

 

Genetic Strategies

   

Order Details

Caspase-3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Application Notes
IHC (Frozen sections) Assay-dependent dilution
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%), Bovine (84%), Dog (89%), Cat (87%), Pig (88%), Rabbit (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.54 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-3 Antibody

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NBP2-15716) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-3.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

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The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP3
Entrez
Uniprot