Caspase-2 Antibody


Western Blot: Caspase-2 Antibody [NBP1-77911] - Transfected human 292 cell lysates. Asterisk indicates a 48 kDa caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against more

Product Details

Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA
0.44 mg/ml

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Caspase-2 Antibody Summary

A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. There is no significant homology to the human form.
Immunogen affinity purified
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  • Western Blot 1:750
  • ELISA 1:1500-1:6000
Application Notes
This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found - be reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used - show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 mice was used - show that this antibody cross reacts with mouse caspase-2.This product was assayed against 1.0 ug of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 - 1:6,000 is suggested for this product. A 1:750 dilution is recommended for immunoblot.
Positive Control
Caspase-2 Lysate (NBP2-64646)
Caspase-2 Lysate (NBP2-65734)

Reactivity Notes

Reactive in mouse, rat

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
0.44 mg/ml
Immunogen affinity purified

Alternate Names for Caspase-2 Antibody

  • caspase-2
  • CASP2
  • CASP-2
  • caspase 2, apoptosis-related cysteine peptidase
  • Caspase2
  • Caspase-2
  • ICH1
  • ICH-1
  • NEDD2
  • NEDD-2
  • PPP1R57


Caspase-2 (Nedd2/ICH-1) is part of a family of aspartate-specific cysteine proteases that participate in programmed cell death, or apoptosis. Caspase-2 is localized to the nucleus, and has been shown to play roles in both positive and negative regulation of apoptosis (1). Upon apoptotic stimulation, pro-caspase-9 is cleaved in a series of steps to produce an 18-kDa large subunit and a 12-kDa small fragment (2). Caspase-2 can be a nuclear initiator caspase, as it has been shown to precede the processing and activation of caspase-8, -9, and -3 (3). Caspase-2 might also act as an effector caspase; in apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of caspase-9 and caspase-3 activation (4).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol CASP2