Caspase-12 Antibody (14F7) - BSA Free

Western Blot: Caspase-12 Antibody (14F7) - BSA Free [NB600-1432] - NRK Rat kidney cell extract was separated on SDS-PAGE and probed with Monoclonal (RAT) AB TO CASPASE-12 CL:14F7. The antibody was developed with HRP APA Rat IgG and an ECL substrate. Antibody concentration 1ug/mL.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, MA
• Clone: 14F7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.0 mg/ml

Caspase-12 Antibody (14F7) - BSA Free Summary

• Immunogen: Recombinant fragment: GPQKICTPSS PSESKRKVEDD EMEVNAGLAH ESHLM LTAPH GLQSSEVQDTL KLCPRDQFCK IKTERAKEIY PVMEKEGRTRL ALIICNKKFDY LFDRDNADTDI LNMQELLENLG YSVVLKENLTA QEMETELMQFA GRPEHQSSDST FLVFMSHGIL EGICGVKHRNK KPDVLHDDTIF KIFNNSNCRSL RNKPKILIMQA CRGRYNGTIWV STNKGIATAD, corresponding to amino acids 95-318 of Mouse Caspase 12.
• Localization: Endoplasmic reticulum
• Specificity: This antibody recognizes the full length and possibly the truncated p20 subunit of Caspase 12.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CASP12
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1-2 ug/ml
• Application Notes: Immunocytochemistry: 4% paraformaldehyde-fixed, paraffin-embedded). Predicted molecular weight: 50 kDa. Antibody may also work in Immunohistochemistry and immunoprecipitation.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Caspase-12 Antibody (14F7) - BSA Free

• Apoptosis Related Cysteine Protease
• CASP 12
• CASP12
• CASP12P1
• Caspase 12 pseudogene 1
• Caspase12
• Caspase-12
• UNQ9415

Background

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene, CASP12, is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP12
• Entrez:
  • 12364(Mouse)
  • 156117(Rat)