Caspase-10/FLICE2 Antibody

Images

 
Western Blot: Caspase 10 Antibody [NBP2-15715] - Sample (30 ug of whole cell lysate) A: Jurkat B: NCI-H929 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Caspase 10 Antibody [NBP2-15715] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Caspase 10 Antibody [NBP2-15715] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Caspase-10/FLICE2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Caspase 10. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-10/FLICE2 Antibody

  • Apoptotic protease Mch-4
  • CASP10
  • CASP-10
  • caspase 10, apoptosis-related cysteine peptidase
  • caspase 10, apoptosis-related cysteine protease
  • Caspase10
  • Caspase-10
  • EC 3.4.22.63
  • FADD-like ICE2
  • FAS-associated death domain protein interleukin-1B-converting enzyme 2
  • FLICE2
  • ICE-like apoptotic protease 4
  • interleukin-1B-converting enzyme 2
  • Mch4
  • MCH4ALPS2

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-10/FLICE2 Antibody (NBP2-15715) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-10/FLICE2.

Caspase 10 - an initiator caspase in the extrinsic death receptor pathway
Apoptosis, also called programmed cell death, is an essential process in development and disease. The signaling networks that carry out apoptosis is consists of a series of endoproteases called caspases which are synthesized as inactive zymogens....  Read full blog post.

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Bioinformatics

Gene Symbol CASP10
Entrez