Caspase-10/FLICE2 Antibody

Images

 
Western Blot: Caspase-10/FLICE2 Antibody [NB100-91683] - Analysis of Caspase 10 (K442) pAb at 1:500 dilution Lane1:Hela whole cell lysate(30ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, phostop) whole cell lysate ...read more
Immunohistochemistry-Paraffin: Caspase-10/FLICE2 Antibody [NB100-91683] - Analyzes of paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: Caspase-10/FLICE2 Antibody [NB100-91683] - Extracts from Hela cell.
Immunohistochemistry-Paraffin: Caspase-10/FLICE2 Antibody [NB100-91683] - Paraffin-embedded human lung carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-91683
    • Availability
      Product Discontinued

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Caspase-10/FLICE2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 410-462 of Human Caspase 10.
Clonality
Polyclonal
Host
Rabbit
Gene
CASP10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Caspase 10 (K442) pAb in extracts from Hela cell.
Immunohistochemistry (IHC) analyzes of Caspase 10 (K442)pAb in paraffin-embedded human lung carcinoma tissue
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-10/FLICE2 Antibody

  • Apoptotic protease Mch-4
  • CASP10
  • CASP-10
  • caspase 10, apoptosis-related cysteine peptidase
  • caspase 10, apoptosis-related cysteine protease
  • Caspase10
  • Caspase-10
  • EC 3.4.22.63
  • FADD-like ICE2
  • FAS-associated death domain protein interleukin-1B-converting enzyme 2
  • FLICE2
  • ICE-like apoptotic protease 4
  • interleukin-1B-converting enzyme 2
  • Mch4
  • MCH4ALPS2

Background

Caspases are a family of intracellular proteases that mediate cell death and are the principal effectors of apoptosis. Caspase 10 (Mch4, ICE-LAP4, FLICE2) plays an important role in apoptosis induced by a variety of inducers such as TNF alpha and Anti-Fas antibody. It is a large prodomain caspase classified together with caspases 2, 8, and 9 as a signaling caspase. Four isoforms of caspase 10 (caspase 10a, 10b, 10c, and 10d) having the same prodomain but different mature large and small subdomain, have been described. Caspase 10 contains two death domains (DED) involved in linking to the death effector domain of the adapter protein FADD and recruiting the complex to TNFR1 and Fas. The inactive procaspase 10 is variably expressed in many tissues and cell lines as a cytosolic protein. The mature form of caspase 10 comprises two subunits, p23/p17 (splice isoforms) and p12. Interestingly, a caspase 9- dependent processing of caspase 10 by caspase 6 in cell-free extracts has recently been suggested. Caspase 10 can cleave and activate caspases 3, 4, 6, 7, 8, and 9. This is followed by cleavage of numerous key proteins, including the nuclear protein PARP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for Caspase-10/FLICE2 Antibody (NB100-91683)

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Diseases for Caspase-10/FLICE2 Antibody (NB100-91683)

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PTMs for Caspase-10/FLICE2 Antibody (NB100-91683)

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Blogs on Caspase-10/FLICE2.

Caspase 10 - an initiator caspase in the extrinsic death receptor pathway
Apoptosis, also called programmed cell death, is an essential process in development and disease. The signaling networks that carry out apoptosis is consists of a series of endoproteases called caspases which are synthesized as inactive zymogens....  Read full blog post.

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Bioinformatics

Gene Symbol CASP10
Entrez