Caspase-1 Recombinant Protein Summary
A recombinant protein corresponding to Human Caspase-1
Source: E. coli.
Amino Acid Sequence: The amino acid sequence of this product is not available.
|Details of Functionality
> 5000 units/mg
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Reconstitute with PBS containing 15% glycerol to a final concentration of 1 unit per ul.
Unit Definition: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
Molecular Weight: large (20 kD) and small (10 kD) subunitsk
Alternate Names for Caspase-1 Recombinant Protein
- caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta
- caspase 1, apoptosis-related cysteine protease (interleukin 1, beta
- EC 3.4.22
- EC 22.214.171.124
- IL-1 beta-converting enzyme
- IL1BC CASP1 nirs variant 1
- interleukin 1-B converting enzyme
- interleukin 1-beta convertase
- Interleukin-1 beta convertase
- Interleukin-1 beta-converting enzyme
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200). The rh-Caspase has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1, gene accession# NP_150634.1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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