Carboxypeptidase B1/CPB1 Antibody

Images

 
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NB120-6627] - Lane 1: Carboxypeptidase B. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carboxypeptidase B primary antibody at 1:1,000 overnight at 4C. Secondary ...read more
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NB120-6627] - Analysis using Biotin conjugate of NB120-6627. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B antibody ...read more

Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
10.0 mg/ml

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Catalog# & Conjugate Size Price

Carboxypeptidase B1/CPB1 Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Carboxypeptidase B [Porcine Pancreas]
Localization
Secreted
Specificity
Carboxypeptidase B
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPB1
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:10 - 1:500

Reactivity Notes

Porcine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
10.0 mg/ml
Purity
Ion exchange chromatography

Alternate Names for Carboxypeptidase B1/CPB1 Antibody

  • carboxypeptidase B
  • carboxypeptidase B1 (tissue)
  • Carboxypeptidase B1
  • CPB
  • CPB1
  • DKFZp779K1333
  • EC 3.4.17
  • EC 3.4.17.2
  • Pancreas-specific protein
  • PASP
  • PCPB
  • procarboxypeptidase B

Background

Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates. This reagent is suited to enable an immunological appraoch to the study of protein and peptide structure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Carboxypeptidase B1/CPB1 Antibody (NB120-6627). (Showing 1 - 2 of 2 FAQ).

  1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
    • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
  2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.
    • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

Secondary Antibodies

 

Isotype Controls

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Diseases for Carboxypeptidase B1/CPB1 Antibody (NB120-6627)

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Bioinformatics

Gene Symbol CPB1
Entrez