Carbonic Anhydrase IX/CA9 Antibody (1G7)

Images

 
Western Blot: Carbonic Anhydrase IX/CA9 Antibody (1G7) [NBP1-47688] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Carbonic Anhydrase IX (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (1G7) [NBP1-47688] - Staining of paraffin-embedded endometrium tissue using anti-Carbonic Anhydrase IXmouse monoclonal antibody.
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (1G7) [NBP1-47688] - HEK293T cells transfected with either pCMV6-ENTRY Carbonic Anhydrase IX.(Red) or empty vector control plasmid (Blue) were immunostained with ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47688
    • Availability
      Product Discontinued

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Carbonic Anhydrase IX/CA9 Antibody (1G7) Summary

Immunogen
Protein expressed in 293T cell transfected with human CA9 expression vector
Marker
Hypoxia Marker
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CA9
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody (1G7)

  • CA9
  • CA-IX
  • CAIXcarbonic anhydrase 9
  • Carbonate dehydratase IX
  • Carbonic Anhydrase IX
  • carbonic anhydrase IXpMW1
  • carbonic dehydratase
  • EC 4.2.1.1
  • G250
  • Membrane antigen MN
  • MN
  • MNRCC-associated antigen G250
  • P54/58N
  • RCC
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-basebalance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Carbonic Anhydrase IX/CA9 Antibody (NBP1-47688). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for the antibody available for cell sorting by CA9. The sorted cells must be cultured for the expansion after the sorting for the following experiments. Do you have the antibodies useful for this?
    • We sell two carbonic anhydrase IX antibodies that have been validated for Flow Cytometry detection of the human protein. These have catalogue numbers NBP1-51691 and NBP1-47688 and can be seen at the following link: carbonic anhydrase IX antibodies. Since both of these products contain sodium azide as a preservative, you are unlikely to be able to culture the cells on following sorting unless you performed a buffer exchange prior to using the antibody. Unfortunately we have no direct experience with either of these products of staining and subsequently expanding cell populations.

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Bioinformatics Tool for Carbonic Anhydrase IX/CA9 Antibody (NBP1-47688)

Discover related pathways, diseases and genes to Carbonic Anhydrase IX/CA9 Antibody (NBP1-47688). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Carbonic Anhydrase IX/CA9 Antibody (NBP1-47688)

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PTMs for Carbonic Anhydrase IX/CA9 Antibody (NBP1-47688)

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Research Areas for Carbonic Anhydrase IX/CA9 Antibody (NBP1-47688)

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Blogs on Carbonic Anhydrase IX/CA9.

Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.

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Bioinformatics

Gene Symbol CA9
Entrez
Uniprot