Recombinant Canine Canis familiaris 1.0101 Strep (N-Term) Protein

Flow Cytometry: Recombinant Canine Canis familiaris 1.0101 Strep (N-Term) Protein [NBP3-06717] - Flow cytometry dot-plot staining pattern of Canis familiaris 1.0101 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (NB100-77913) FITC and anti-human CD203c PE antibodies.

ELISA: Recombinant Canine Canis familiaris 1.0101 Strep (N-Term) Protein [NBP3-06717] - ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen Canis familiaris 1.0101 and allergen-specific human plasma IgG4 antibodies of Canis familiaris positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Canis familiaris 1.0101.

SDS-Page: Recombinant Canine Canis familiaris 1.0101 Strep (N-Term) Protein [NBP3-06717] - Recombinant allergen Canis familiaris 1.0101 purity verification. 5 ug of Canis familiaris 1.0101 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

• Reactivity: Ca
• Applications: WB, ELISA, Flow

Recombinant Canine Canis familiaris 1.0101 Strep (N-Term) Protein Summary

• Description: A recombinant protein with a N-Terminal Strep-tag corresponding to 169 amino acids of Canis familiaris 1.0101
  
  

  Source: E.coli

  Accession Number: NP_001003190.1
  
  Store product aliquots at -20C to -70C. Use stored aliquots within 6 months.

• Preparation Method: The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by HPLC

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Western Blot
• Theoretical MW: 18.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0
• Preservative: No Preservative
• Purity: >95%, by HPLC
• Reconstitution Instructions: Reconstitute with sterile deionized water with or without azide.

Alternate Names for Recombinant Canine Canis familiaris 1.0101 Strep (N-Term) Protein

• rCan f 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.