Canine 20S Immunoproteasome Protein, CF

Images

 
There are currently no images for 20S Immunoproteasome (E-377).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity CaSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Canine 20S Immunoproteasome Protein, CF Summary

Details of Functionality
The Canine 20S Immunoproteasome is able to degrade substrates in an ATP-independent manner. It can be activated chemically with SDS (0.035%) or by the addition of PA28. Reaction conditions will need to be optimized for each specific application. We recommend an initial Canine 20S Immunoproteasome concentration of 0.5-5 nM.
Source
Canine spleen-derived 20S Immunoproteasome protein
Protein/Peptide Type
Natural Enzymes
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
700 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (X μM) in 50 mM HEPES pH 7.6, 100 mM NaCl, 1mM DTT

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Canine 20S Immunoproteasome Protein, CF

  • 20S Immunoproteasome

Background

The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi-complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged into four stacked rings (1,2). However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, 2, and 5, in the two interior rings of the 20S Proteasome are replaced by three IFN-gamma-inducible catalytic subunits: beta 1i/LMP2, beta 2i/LMP7, and beta 5i/MECL-1 (3). The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha/beta, or the PA28 gamma regulatory complexes (3,4). 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation (3,5,6). Selective inhibition of 20S Immunoproteasome proteolytic activity using small molecule inhibitors is being examined for therapeutic intervention in cancer and inflammatory diseases (7).    

This protein has been purified from canine spleen.

  1. Kim, H.M. et al. (2011) Biochim. Biophys. Acta 1809:67.
  2. Xie, Y. (2010) J. Mol. Cell Biol. 2:308.
  3. Kloetzel, P.M. (2001) Nat. Rev. Mol. Cell Biol. 2:179.
  4. Stadtmueller, B.M. & C.P. Hill (2011) Mol. Cell 41:8.
  5. Cascio, P. et al. (2001) EMBO J. 20:2357.
  6. Ferrington, D.A. & D.S. Gregerson (2012) Prog. Mol. Biol. Transl. Sci. 109:75.
  7. Lee, W. & K.B. Kim (2011) Curr. Top. Med. Chem. 11:2923.

Publications for 20S Immunoproteasome (E-377) (0)

There are no publications for 20S Immunoproteasome (E-377).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 20S Immunoproteasome (E-377) (0)

There are no reviews for 20S Immunoproteasome (E-377). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for 20S Immunoproteasome (E-377) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Blogs on 20S Immunoproteasome

There are no specific blogs for 20S Immunoproteasome, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Canine 20S Immunoproteasome Protein, CF and receive a gift card or discount.