CaMKK2 Antibody

Images

 
Western Blot: CaMKK2 Antibody [NBP2-15687] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CaMKK beta antibody [N2C1], Internal diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CaMKK2 Antibody [NBP2-15687] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: CaMKK2 Antibody [NBP2-15687] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.
Western Blot: CaMKK2 Antibody [NBP2-15687] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: CaMKK2 Antibody [NBP2-15687] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CaMKK2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CaMKK beta. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAMKK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CaMKK2 Antibody

  • calcium/calmodulin-dependent protein kinase kinase 2
  • calcium/calmodulin-dependent protein kinase kinase 2, beta
  • Calcium/calmodulin-dependent protein kinase kinase beta
  • CaM-kinase kinase 2
  • CaM-kinase kinase beta
  • CaM-KK 2
  • CAMKK beta protein
  • CaMKK beta
  • CaM-KK beta
  • CAMKK
  • CAMKKBCaMKK 2
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0787calcium/calmodulin-dependent protein kinase beta
  • MGC15254

Background

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct isoforms have been identified for this gene. Additional splice variants have been described but their full-length nature has not been determined. The identified isoforms exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CAMKK2
Uniprot