CaMKII alpha/delta [p Thr286] Antibody

Images

 
Western Blot: CaMKII alpha/delta [p Thr286] Antibody [NBP1-51462] - analysis of CaMKII in human melanoma cells using anti-CaMKII alpha/delta [p Thr286] antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: CaMKII alpha/delta [p Thr286] Antibody [NBP1-51462] - Human brain tissue.
Western Blot: CaMKII alpha/delta [p Thr286] Antibody [NBP1-51462] - Extracts from K562 cells.

Product Details

Summary
Product Discontinued
View other related CaMKII alpha/delta Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51462
    • Availability
      Product Discontinued

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CaMKII alpha/delta [p Thr286] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human CaMKIIalpha around the phosphorylation site of Threonine 286.
Modification
p Thr286
Specificity
p-CaMKII alpha/delta (T286) pAb detects endogenous levels of CaMKII alpha/delta protein only when phosphorylated at T286.
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of p-CaMKIIalpha /Delta (T286) pAb in extracts from K562 cells.
Immunohistochemistry (IHC) analyzes of p-CaMKIIalpha /Delta (T286) pAb in paraffin-embedded human brain tissue.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-51462 in the following applications:

Publications
Read Publication using NBP1-51462.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CaMKII alpha/delta [p Thr286] Antibody

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • CaM kinase II alpha subunit
  • CaM kinase II subunit alpha
  • CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
  • CaMK-II alpha subunit
  • CaMK-II subunit alpha
  • CaMKIINalpha
  • CaM-kinase II alpha chain
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CaMKII alpha/delta Antibody (NBP1-51462)(1)

Review for CaMKII alpha/delta Antibody (NBP1-51462) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-51462:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot CaMKII alpha/delta NBP1-51462
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reviewed by:
Amanpreet Kaur
WB Human 03/16/2015
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Summary

ApplicationWestern Blot
Sample TestedMelanoma cells
SpeciesHuman

Blocking

Blocking Details5% BSA for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:500 dilution in 5% BSA, membrane was incubated ovrenight at 4C.

Secondary Antibody

Secondary DescriptionGoat anti-rabbit (1:2000) in 5% nonfat dry milk
Secondary Manufacturer Cat#sc-2004
Secondary Concentration(1:2000) in 5% nonfat dry milk

Details

Detection NotesECL2 (Thermo Scientific) for 5 minutes.

Comments

Comments1. I also tried harvesting my samples in RIPA but I did not get any bands in those samples.2. The antibody can detect multiple different isoforms but the alpha isoform shows up first in my cell lines.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CaMKII alpha/delta Antibody (NBP1-51462) (0)

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Secondary Antibodies

 

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Diseases for CaMKII alpha/delta Antibody (NBP1-51462)

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PTMs for CaMKII alpha/delta Antibody (NBP1-51462)

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Recent Reviews

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5
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4
1
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1
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Amanpreet Kaur
03/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CAMK2A
Entrez
Uniprot