Calpain 2 Antibody (1F4)

Images

 
Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpain 2 antibody, and then ...read more
Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Hela cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Jurkat cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, Flow
Clone
1F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Calpain 2 Antibody (1F4) Summary

Immunogen
Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CAPN2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Calpain 2 Antibody (1F4)

  • Calcium-activated neutral proteinase 2
  • calpain 2, (m/II) large subunit
  • calpain 2, large [catalytic] subunit
  • calpain 2, large subunit
  • Calpain large polypeptide L2
  • Calpain M-type
  • calpain, large polypeptide L2
  • calpain-2 catalytic subunit
  • Calpain-2 large subunit
  • CANP 2
  • CANPL2CANP2
  • CANPml
  • EC 3.4.22
  • EC 3.4.22.53
  • FLJ39928
  • M-calpain
  • mCANP
  • millimolar-calpain

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CAPN2
Entrez