Calpain 1 Antibody (9A4H8D3)

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Western Blot: Calpain 1 Antibody (9A4H8D3) [NB120-3589] - Analysis of 15ug of HeLa cell lysate (lane 2), 15ug of A431 cell lysate (lane 3) and 7ul of MagicMark™ XP Western Protein Standard (lane 1, Product # ...read more
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (9A4H8D3) [NB120-3589] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ...read more
Immunohistochemistry-Paraffin: Calpain 1 Antibody (9A4H8D3) [NB120-3589] - Normal biopsies of deparaffinized human tonsil tissue.
Western Blot: Calpain 1 Antibody (9A4H8D3) [NB120-3589] - Analysis of mouse lung extract.
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (9A4H8D3) [NB120-3589] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ...read more
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (9A4H8D3) [NB120-3589] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ...read more
Immunohistochemistry-Paraffin: Calpain 1 Antibody (9A4H8D3) [NB120-3589] - Normal biopsies of deparaffinized human kidney tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-3589
    • Availability
      Product Discontinued

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Calpain 1 Antibody (9A4H8D3) Summary

Immunogen
Purified bovine skeletal muscle 80 kDa mu-calpain subunit.
Epitope
This antibody recognizes an epitope between amino acids 465-520 (domain III) of human Mu Calpain.
Localization
Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Specificity
Detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, mouse lung, pig cultured cells and hamster and rabbit samples. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CAPN1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
IHC-Fr usage was reported in scientific literature (PMID; 24413151). WB: Detects an approx. 80 kDa protein representing Mu Calpain from human platelets and erythrocytes. ICC: Staining of Mu Calpain in LLC-PK1 cells results in diffuse cytoplasmic staining.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Calpain 1 Lysate (NBL1-08677)
Publications
Read Publication using
NB120-3589 in the following applications:

Reactivity Notes

Arthropod reactivity reported in scientific literature (PMID: 18722464). Sheep reactivity reported in scientific literature (PMID: 26059039). Yeast reactivity reported in scientific literature (PMID: 16303960). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Calpain 1 Antibody (9A4H8D3)

  • Calcium-activated neutral proteinase 1
  • calpain 1, (mu/I) large subunit
  • Calpain mu-type
  • calpain, large polypeptide L1
  • calpain-1 catalytic subunit
  • Calpain-1 large subunit
  • CANP 1
  • CANP
  • CANPL1EC 3.4.22.52
  • Cell proliferation-inducing gene 30 protein
  • cell proliferation-inducing protein 30
  • EC 3.4.22
  • micromolar-calpain
  • muCANPCANP1
  • muCL

Background

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calpain 1 Antibody (NB120-3589)(1)

We have publications tested in 1 confirmed species: Zebrafish.

We have publications tested in 1 application: IHC-Fr.


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IHC-Fr
(1)
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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Positive Control Lysate(s)

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Bioinformatics

Gene Symbol CAPN1
Entrez
Uniprot