Caldesmon/CALD1 Antibody (C21)

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Summary
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    • Catalog Number
      NB120-11016
    • Availability
      Product Discontinued

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Caldesmon/CALD1 Antibody (C21) Summary

Immunogen
Purified chicken gizzard caldesmon.
Epitope
The antibody is directed to an epitope near the carboxy-terminusCa2+/Calmodulin and F-actin binding site of caldesmon.
Localization
Cytoplasmic
Specificity
The antibody is directed against an epitope near the carboxy-terminus, Ca2+/calmodulin and F-actin binding site of caldesmon. Using ELISA, the antibody is able to inhibit caldesmon binding to Ca2+/calmodulin and F-actin. By immunoblotting, the antibody shows broad species reactivity to non-muscle caldesmon of mammalian cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CALD1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Radioimmunodiffusion
Application Notes
DID: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. The minimum antibody titer of 1/2500 was determined in an ELISA with turkey gizzard or pig stomach caldesmon coated at 10 g/ml on polyvinyl microtiter plates.IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Caldesmon occurs in both a high molecular weight (120-150 KD) and a low molecular weight (60-80KD) form, depending on the tissue in which it is located.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Rat, Gerbil, Pig and Turkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Raw ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Caldesmon/CALD1 Antibody (C21)

  • CAD
  • CALD1
  • caldesmon 1
  • Caldesmon
  • CDM
  • CDMH-CAD
  • HCAD
  • LCAD
  • L-CAD
  • MGC21352
  • NAG22

Background

Monoclonal anti-Caldesmon may be used for the study of the involvement of caldesmon in contraction, cell movement, shape change, exocytosis and endocytosis. Caldesmon is an actin interacting and calmodulin binding protein found in smooth muscle and other cell types. Caldesmon occurs in both a high molecular weight (120-150 kDa) and a low molecular weight (71-80 kDa) form, depending on the tissue in which it is located. Caldesmon plays a major role in the regulation of smooth muscle and non-muscle contractile events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CALD1
Entrez