Calcein, AM Solution


Immunocytochemistry/ Immunofluorescence: Calcein, AM Solution [NBP2-31157] - Hela Cell stained
Calcein, AM Solution [NBP2-31157] - MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# 89202). Images acquired every 3 seconds and total elapsed more
Calcein, AM Solution [NBP2-31157] - Calcein AM Ultra-pure Grade is guaranteed to be at least 95% pure. This HPLC trace shows that for this particular lot, the purity is at > 98%.

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Calcein, AM Solution Summary

Calcein AM is a viability dye that can be used in permeabilized cells. Upon uptake by a live cell, Calcein AM is cleaved by intracellular esterases resulting in fluorescence. Dead cells will not have sufficient esterase activity and will not fluoresce. In this way, dead cells may be identified by their low Calcein signal. Calcein is efficiently excited by the 488nm blue laser and emits at 516 nm (FL1 on a standard 4 color flow cytometer).


Application Notes
Calcein AM should be used at as low a final working concentrations as possible to minimize potential artifacts (usually between 1 and 10 uM). Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes.

Packaging, Storage & Formulations

Store at -20C in the dark. Avoid freeze-thaw cycles.
No Preservative
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for Calcein, AM Solution

  • Calcein AM
  • Calcein
  • Calcein, AM


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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