Calcein, AM Solution

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Flow Cytometry: Calcein, AM Solution [NBP2-31157] - Hela Cell stained

Product Details

Summary
Applications Flow
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

Calcein, AM Solution Summary

Description
Calcein AM is a viability dye that can be used in permeabilized cells. Upon uptake by a live cell, Calcein AM is cleaved by intracellular esterases resulting in fluorescence. Dead cells will not have sufficient esterase activity and will not fluoresce. In this way, dead cells may be identified by their low Calcein signal. Calcein is efficiently excited by the 488nm blue laser and emits at 516 nm (FL1 on a standard 4 color flow cytometer).

Applications/Dilutions

Application Notes
Calcein AM should be used at as low a final working concentrations as possible to minimize potential artifacts (usually between 1 and 10 uM). Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes.

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Buffer
DMSO
Preservative
No Preservative
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for Calcein, AM Solution

  • Calcein AM
  • Calcein
  • Calcein, AM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Product General Protocols

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