CA19-9/Sialyl Lewisa Antibody (SPM588) - IHC-Prediluted

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P
Clone
SPM588
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

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CA19-9/Sialyl Lewisa Antibody (SPM588) - IHC-Prediluted Summary

Immunogen
Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le).
Localization
Cytoplasmic
Marker
GI Tumor Marker
Specificity
CA19-9, a carbohydrate epitope expressed on a high MW (>400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This MAb is superb for staining of formalin-fixed, paraffin-embedded tissues.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
Application Notes
Molecular weight of antigen: >400kDa Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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