C20orf85 Overexpression Lysate (Native)

Images

 
Western Blot: C20orf85 Overexpression Lysate (Adult Normal) [NBL1-08382] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C20orf85.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

C20orf85 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206670 and based on accession number NM_178456. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 20 open reading frame 85 (C20orf85), mRNA.
Gene
C20ORF85

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for C20orf85

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for C20orf85 Overexpression Lysate (Native)

  • bA196N14.1
  • chromosome 20 open reading frame 85
  • hypothetical protein LOC128602
  • LLC1
  • low in lung cancer 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31308
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1414
Species: Hu
Applications: WB, PEP-ELISA
NB100-56598
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P
NBP1-87679
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-50179
Species: Hu
Applications: WB, Flow, Func, ICC/IF, IP, CyTOF-ready
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP2-24530
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P, Flow-IC
NBP1-89942
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-90149
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBL1-08382
Species: Hu
Applications: WB

Publications for C20orf85 Lysate (NBL1-08382) (0)

There are no publications for C20orf85 Lysate (NBL1-08382).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for C20orf85 Lysate (NBL1-08382) (0)

There are no reviews for C20orf85 Lysate (NBL1-08382). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for C20orf85 Lysate (NBL1-08382) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional C20orf85 Products

Bioinformatics Tool for C20orf85 Lysate (NBL1-08382)

Discover related pathways, diseases and genes to C20orf85 Lysate (NBL1-08382). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for C20orf85 Lysate (NBL1-08382)

Discover more about diseases related to C20orf85 Lysate (NBL1-08382).
 

Pathways for C20orf85 Lysate (NBL1-08382)

View related products by pathway.

PTMs for C20orf85 Lysate (NBL1-08382)

Learn more about PTMs related to C20orf85 Lysate (NBL1-08382).

Blogs on C20orf85

There are no specific blogs for C20orf85, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF
IL-2 [Unconjugated]
202-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our C20orf85 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol C20ORF85