C1q R1/CD93 Antibody (R139)

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Flow Cytometry: C1q R1/CD93 Antibody (R139) [NBP1-43378] - Analysis using the Biotin conjugate of NBP1-43378. Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IP
Clone
R139
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

C1q R1/CD93 Antibody (R139) Summary

Immunogen
The immunogen for this antibody was CD93.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD93
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
This R139 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for C1q R1/CD93 Antibody (R139)

  • AA4 Antigen
  • C1q R1
  • C1q receptor 1
  • C1q Rp
  • C1q/MBL/SPA receptor
  • C1qR(P)
  • C1qR1
  • C1qRp
  • CD93 antigenC1qR
  • CD93 molecule
  • CD93
  • CDw93C1QR1
  • Collectin Receptor
  • Complement component 1 q subcomponent receptor 1
  • complement component 1, q subcomponent, receptor 1
  • complement component C1q receptor
  • dJ737E23.1
  • ECSM3
  • Ly68
  • Matrix-remodeling-associated protein 4
  • matrix-remodelling associated 4
  • MXRA4

Background

The monoclonal antibody R139 recognizes human CD93, also known as C1qRp. The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A. CD93 is predicted to play a role in the clearance of apoptotic cells. Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells. Expression on DCs is downregulated upon maturation. Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors. The monoclonal antibody R139 blocks C1q-mediated enhancement of phagocytosis. CD93 can be shed from the cell surface. This phenomenon can be measured using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA. The epitope for R139 resides in the EGF domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43378AF405
Alexa Fluor 488 Labeled NBP1-43378AF488
Alexa Fluor 647 Labeled NBP1-43378AF647
Alexa Fluor 700 Labeled NBP1-43378AF700
Allophycocyanin Labeled NBP1-43378APC
DyLight 350 Labeled NBP1-43378UV
DyLight 405 Labeled NBP1-43378V
DyLight 488 Labeled NBP1-43378G
DyLight 680 Labeled NBP1-43378FR
DyLight 755 Labeled NBP1-43378IR
PE Labeled NBP1-43378PE
PerCP Labeled NBP1-43378PCP

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Bioinformatics

Gene Symbol CD93
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