C17orf39 Overexpression Lysate (Native)

Images

 
Western Blot: C17orf39 Overexpression Lysate (Adult Normal) [NBL1-08233] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C17orf39.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

C17orf39 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207341 and based on accession number NM_024052. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 17 open reading frame 39 (C17orf39), mRNA.
Gene
GID4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
33.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for C17orf39

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for C17orf39 Overexpression Lysate (Native)

  • C17orf39
  • chromosome 17 open reading frame 39
  • GID complex subunit 4, VID24 homolog (S. cerevisiae)
  • hypothetical protein LOC79018
  • MGC3048
  • VID24

Background

The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol GID4