c11orf59/LAMTOR1 Antibody

Images

 
Western Blot: c11orf59/LAMTOR1 Antibody [NBP1-71689] - Analysis of c11orf59 in human liver protein.
Immunocytochemistry/ Immunofluorescence: c11orf59/LAMTOR1 Antibody [NBP1-71689] - C11orf59 antibody was tested at 1:50 in HepG2 cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).
Immunohistochemistry: c11orf59/LAMTOR1 Antibody [NBP1-71689] - Analysis of C11orf59 in mouse pancreas.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

c11orf59/LAMTOR1 Antibody Summary

Immunogen
A genomic peptide made to a C-terminal region of the human c11orf59/LAMTOR1 protein (within residues 50-250). [Swiss-Prot Q6IAA8]
Localization
Late endosome membrane; Lipid-anchor; Cytoplasmic side. Lysosome membrane; Lipid-anchor; Cytoplasmic side. Cell membrane
Predicted Species
Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMTOR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:25-1:100
  • Immunohistochemistry 1:25-1:100
  • Immunohistochemistry-Paraffin 1:25-1:100
Application Notes
This c11orf59 antibody is useful for IHC-P, ICC/IF and Western blot, where a band is seen ~22 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
Liver Lysate (NB820-59233)

Reactivity Notes

Human, mouse and rat. Immunogen has 98% identity to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for c11orf59/LAMTOR1 Antibody

  • chromosome 11 open reading frame 59
  • FLJ20625
  • LAMTOR1
  • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1
  • late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
  • Lipid raft adaptor protein p18
  • p18
  • p27kip1 releasing factor from RhoA
  • p27Kip1-releasing factor from RhoA
  • p27RF-RhoC11orf59
  • PDRO
  • PDROPdro
  • Protein associated with DRMs and endosomes
  • ragulator complex protein PDRO
  • Ragulator1
  • RhoA activator C11orf59

Background

c11orf59/LAMTOR1 (ragulator complex protein LAMTOR1 or PDRO), a part of Ragulator complex which contains LAMTOR1-5, is a relatively novel lapidated protein adaptor of the lysosomal membrane which senses and integrates a diverse array of cellular signals, with mTOR kinase activity within the complex being influenced by a variety of nutrients e.g. amino acids, glucose etc., oxygen status, cellular energy levels, and many secreted growth factors such as cytokines and hormones, including insulin. Ragulator is activated by amino acids through a mechanism involving lysosomal V-ATPase, and it functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Ragulator (activated) and Rag GTPases function as a scaffold that recruits mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes and is also required for late endosomes/lysosomes biogenesis. It regulates both the recycling of receptors through endosomes and MAPK signaling pathway through recruitment of some of its components to late endosomes. It implicates in cholesterol homeostasis for regulating LDL uptake and cholesterol release from late endosomes/lysosomes, and may play a role in RHOA activation. LAMTOR1 deregulation is observed in certain pathologies including cancer, diabetes and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c11orf59/LAMTOR1 Antibody (NBP1-71689) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LAMTOR1
Entrez
Uniprot