c-jun [p Thr239] Antibody

Images

 
Western Blot: c-jun Antibody [NB100-92506] - Western blot (WB) analysis of c-jun (T239) antibody in extracts from HeLa cells treated with UV.
Immunohistochemistry-Paraffin: c-jun Antibody [NB100-92506] - Paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92506
    • Availability
      Product Discontinued

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c-jun [p Thr239] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Threonine 239.
Modification
p Thr239
Clonality
Polyclonal
Host
Rabbit
Gene
JUN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:500-1:200
Application Notes
Western blot (WB) analysis of c-jun (T239) pAb in extracts from HeLa cells treated with UV..
Immunohistochemistry (IHC) analyzes of c-jun (T239) pAb in paraffin-embedded human breast carcinoma tissue
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
c-jun Lysate (NBL1-12116)

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-jun [p Thr239] Antibody

  • Activator protein 1
  • AP1
  • AP-1
  • cJun
  • c-Jun
  • enhancer-binding protein AP1
  • Jun activation domain binding protein
  • jun oncogene
  • jun proto-oncogene
  • JUN
  • Proto-oncogene c-Jun
  • transcription factor AP-1
  • V-jun avian sarcoma virus 17 oncogene homologp39
  • v-jun sarcoma virus 17 oncogene homolog (avian)
  • v-jun sarcoma virus 17 oncogene homolog

Background

Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, but the Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is deactivated upon phosphorylation induced by the cAMPdependent protein kinase A (PKA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on c-jun.

Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration
By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

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Bioinformatics

Gene Symbol JUN
Entrez