BubR1 Antibody

Images

 
Western Blot: BubR1 Antibody [NBP1-19555] - Analysis of BUB1B (T368) pAb at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate
Western Blot: BubR1 Antibody [NBP1-19555] - extracts from HeLa cells treated with H2O2 100uM 30

Product Details

Summary
Product Discontinued
View other related BubR1 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-19555
    • Availability
      Product Discontinued

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BubR1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 341-390 of Human BUB1B.
Specificity
BUB1B (T368) pAb detects endogenous levels of BUB1B protein.
Clonality
Polyclonal
Host
Rabbit
Gene
BUB1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of ubR1 (T368) pAb in extracts from HeLa cells treated with H2O2 100uM 30'.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-19555 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BubR1 Antibody

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

The N-end rule relates the in vivo half-life of a protein to the composition of its N-terminal residues. The N-end rule pathway is part of the ubiquitin system, which involves a three-step mechanism. Proteins targeted for degradation are bound on their N-terminal residue by Ubr1 (also designated E3 alpha and N-recognin), which catalyzes the covalent attachment of ubiquitin to the protein substrate. Two zinc finger domains and the RING-H2 finger domain of Ubr1 are essential for substrate recognition. Ubr1 is located on mouse chromosome 2 and on human chromosome 15 in the syntenic region. Ubr1 is a 220 kDa protein that is ubiquitously expressed in adult mouse, with the highest expression detected in skeletal muscle and heart. In mouse embryo, Ubr1 is primarily expressed in the branchial arches and in the tail and limb buds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BubR1 Antibody (NBP1-19555)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(2)
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Mouse
(2)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BUB1B
Entrez